The thermodynamic basis for the partial control of oxidative phosphorylation by the adenine-nucleotide translocator

Table 1. The eflect of norvaline on citrulline synthesis Rat liver mitochondria (3.0 mg of protein/ml) were incubated in a medium containing lOOmM-KcI, 50mM-Tris/HCI, 1 mMEGTA, 1 mM-ATP, 20m~-succinate, 2pg of rotenone/ml, 5 mM-potassium phosphate, 16.6 mM-KHCO,, 10 mM-NH,CI and 3 m~-[~‘Clornithine (0.5 pCi/ml). After 4 min, mitochondria were separated from the suspending medium by silicone-oil centrifugation. Carbamoyl phosphate (CP) and ATP were determined in the neutralized HCIO, extracts. Parallel incubations with I “Clsucrose and ’H,O were carried out in order to determine the intramitochondrial ornithine concentration after separation of ornithine and citrulline by column chromatography.